On-chip Sort CONSUMABLES : REAGENTS

On-chip Biotechnologies supplies various reagents to assist you in sample preparation, analysis and sorting on On-chip Sort, and single cell dispensing on On-chip SPiS. All of the below products are confirmed to be non-toxic to most cells under normal conditions.

Anti-cell adhesion reagent

Cell-adhesion prevention:

Our special reagents prevent cell adhesion to surfaces, such as walls of tubes and pipiette tips. This reagent works by coating surfaces prior to experiments or by mixing the reagent in media, and thus it will be useful in various cell-based experiments.
A significant reduction in cell loss for highly sticky cells like stem cells and cancer cells!

Sheath fluid adjustment:

The use of our anti-cell adhesion reagent in sheath fluid allows users to handle cells without cell loss during analysis and sorting. Simply add a small amount of our reagent in media of your preference, and the mixture can be used as sheath fluid.

Through Path Plus 40×

Through Path Plus 40x is a reagent added to culture medium or buffer such as PBS to be used as cell suspension fluid and sheath fluid. It allows microchannel and reservoirs of the microfluidic chip to be coated to prevent cell adhesion and thus cell loss.

Product name	Through Path Plus 40×
Catalog number	2001004-6
Quantity	10 mL x 6 bottles
Usage	Dilute 40-folds with culture medium or buffer (e.g., PBS)
Shipping condition	Room temperature
Storage condition	4℃ (use within 1 month after opening)

Sheath fluid

Ready-to-use sheath fluids are available for purchase. These contain the anti-cell adhesion reagent, Through Path Plus. Sheath fluids are used to run the cell sorter.

On-chip Sheath

On-chip Sheath is a ready-to-use serum-free cell culture medium to suspend cells and used as sheath fluid. It contains Through Path Plus to allow coating of microchannel and reservoirs of the microfluidic chip to prevent cell adhesion and thus cell loss.

Product name	On-chip Sheath
Catalog number	2001012
Quantity	200 mL
Usage	Use as is
Shipping condition	Room temperature
Storage condition	4℃ (use within 1 month after opening)

On-chip T buffer

On-chip T buffer is a ready-to-use PBS-based buffer to suspend cells and used as sheath fluid. It contains Through Path Plus to allow coating of microchannel and reservoirs of the microfluidic chip, pipette tips and tubes to prevent cell adhesion, and thus cell loss.

Product name	On-chip T buffer
Catalog number	2001014
Quantity	500 mL
Usage	Use as is
Shipping condition	Room temperature
Storage condition	4℃ (use within 1 month after opening)

Sample buffer

We supply buffers used to suspend cells. Those buffers contain additives to reduce the rate of cell sedimentation. They are ready-to-use, and they come in 1x solution, 2x solution and powder form.

On-chip Sample Buffer

On-chip Sample Buffer is a ready-to-use serum-free cell culture medium used to suspend cells for sorting. It contains Through Path Plus to allow coating of microchannel and reservoirs of the microfluidic chip to prevent cell adhesion and thus cell loss, and viscosity agent for prolonged cell suspension.

Product name	On-chip Sample Buffer
Catalog number	2001013
Quantity	50 mL
Usage	Add directly to cell pellet
Shipping condition	Room temperature
Storage condition	4℃ (use within 1 month after opening)

On-chip Sample Buffer 2×

Product name	On-chip Sample Buffer 2×
Catalog number	2001015
Quantity	50 mL
Usage	Dilute 1:1 with sample
Shipping condition	Room temperature
Storage condition	4℃ (use within 1 month after opening)

On-chip Sample Buffer Production kit

A kit to produce Sample buffer with your preferred media. This is best for when using sensitive cells or special medium. It does not contain Through Path, and it can be added as required.

Product name	On-chip Sample Buffer Production kit
Catalog number	2001011
Quantity	3 packs to produce 15 mL Sample buffer each
Usage	Dissolve this product in 15mL of medium to be used as Sample buffer, or dissolve in 7.5mL of medium to be used as Sample buffer 2x equivalent.
Shipping condition	Room temperature
Storage condition	4℃ (use within 2 weeks after opening)