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Phage detection and isolation with W/O Droplet
Miu Hoshino, Naohiro Noda
National Institute of Advanced Industrial Science and Technology (AIST)
Introduction: Phages are viruses that infect bacteria. Recently, phages have begun to attract attention for use as microbial control agents in fields such as medicine, agriculture, and food safety. Screening and isolation of phages from environmental samples is still challenging due to the low-throughput of the existing method, plaque assay. Plaque assay requires the use of agarose plates, which may be problematic when using bacteria that degrade, or cannot grow on agar. A novel assay using water-in-oil droplets was developed to overcome these obstacles.