PUBLICATIONS - Microfluidic Chip-Based Gentle Cell Sorter, Single Cell & Cluster Dispenser | On-chip Bio

PUBLICATIONS

Articles and Presentations Related to On-chip

Journal Articles

A single-cell transcriptomic and anatomic atlas of mouse dorsal raphe Pet1 neurons
Benjamin W Okaty, Nikita Sturrock, Yasmin Escobedo Lozoya, YoonJeung Chang, Rebecca A Senft, Krissy A Lyon, Olga V Alekseyenko, and Susan M Dymecki.
eLife, 9: e55523, June 2020.

 

Successful selection of mouse sperm with high viability and fertility using microfluidics chip cell sorter
Satohiro Nakao, Toru Takeo, Hitomi Watanabe, Gen Kondoh and Naomi Nakagata .
Scientific Reports, 10: 8862, June 2020.

 

Establishing typical values for hemocyte mortality in individual California mussels, Mytilus californianus
Nicole E Moyen, Paul A Bump, George N Somero, and Mark W Denny.
Fish and Shellfish Immunology, 100: 70-79, May 2020.

 

Detection of circulating sarcoma tumor cells using a microfluidic chip-type cell sorter
Nobuhiko Hasegawa, Ikuko Takeda Nakamura, Toshihide Ueno, Shinya Kojima, Masahito Kawazu, Keisuke Akaike, Taketo Okubo, Tatsuya Takagi, Yoshiyuki Suehara, Takuo Hayashi, Tsuyoshi Saito, Kazuo Kaneko, Hiroyuki Mano, and Shinji Kohsaka.
Scientific Reports, 9: 20047, December 2019.

 

Development of a Highly Sensitive Technique for Capturing Renal Cell Cancer Circulating Tumor Cells
Michio Naoe, Chiho Kusaka, Mika Ohta, Yuki Hasebe, Tsutomu Unoki, Hideaki Shimoyama, Takehiko Nakasato, Kazuhiko Oshinomi, Jun Morita, Kohzo Fuji, Yoshio Ogawa, Mana Tsukada, Masataka Sunagawa, and Hikaru Ishii.
Diagnostics, 96(3), August 2019.

 

Fluorescent nucleic acid probe in droplets for bacterial sorting (FNAP-sort) as a high-throughput screening method for environmental bacteria with various growth rates
Yuri Ota, Kanako Saito, Taeko Takagi, Satoko Matsukura, Masamune Morita, Satoshi Tsuneda, and Naohiro Noda.
PLOS ONE, 14(4), April 2019.

 

Isolation and molecular analysis of circulating tumor cells from lung cancer patients using a microfluidic chip type cell sorter
Masaru Watanabe, Hirotsugu Kenmotsu, Ryo Ko, Kazushige Wakuda, Akira Ono, Hisao Imai, Tetsuhiko Taira, Tateaki Naito, Haruyasu Murakami, Masato Abe, Masahiro Endo, Takashi Nakajima, Toshiaki Takahashi, and Yasuhiro Koh.
Cancer Science, 109(8), July 2018.

 

Ecel1 knockdown with an AAV2-mediated CRISPR/Cas9 system promotes optic nerve damage-induced RGC death in the mouse retina
Kota Sato, Yukihiro Shiga, Yurika Nakagawa, Kosuke Fujita, Koji M. Nishiguchi, Hiroshi Tawarayama, Namie Murayama, Shigeto Maekawa, Takeshi Yabana, Kazuko Omodaka, Shota Katayama, Qiwei Feng, Satoru Tsuda, and Toru Nakazawa.
Investigative Ophthalmology & Visual Science, 59(10), July 2018.

 

Monitoring circulating tumor DNA revealed dynamic changes in KRAS status in patients with metastatic colorectal cancer
Yuji Takayama,1 Koichi Suzuki, Yuta Muto,1 Kosuke Ichida, Taro Fukui, Nao Kakizawa, Hideki Ishikawa, Fumiaki Watanabe, Fumi Hasegawa, Masaaki Saito, Shingo Tsujinaka, Kazushige Futsuhara, Yasuyuki Miyakura, Hiroshi Noda, Fumio Konishi, and Toshiki Rikiyama.
Oncotarget, 9(36): 24398–24413, May 2018.

 

「血中循環腎癌細胞回収についてOn-chip SortⓇとClearCell FXⓇシステムとの比較検討」
松井 祐輝, 直江 道夫, 太田 実香, 下山 英明, 鵜木 勉, 中里 武彦, 押野見 和彦, 森田 順, 前田 佳子, 冨士 幸蔵, 小川 良雄.
昭和学士会雑誌/78巻2号/書誌, 2018

 

Nascent Induced Pluripotent Stem Cells Efficiently Generate Entirely iPSC-Derived Mice while Expressing Differentiation-Associated Genes
Bhishma Amlani, Yiyuan Liu, Taotao Chen, Ly-Sha Ee, Peter Lopez, Adriana Heguy, Effie Apostolou, Sang Yong Kim, and Matthias Stadtfeld.
Cell Report, 22(4): 876–884, January 2018.

 

Microproteomics with microfluidic-based cell sorting: Application to 1000 and 100 immune cells
Kie Kasuga, Yasutake Katoh, Keisuke Nagase, and Kazuhiko Igarashi.
Proteomics, 17(13-14): 1600420, July 2017.

 

血中がん細胞診断(血中循環腫瘍細胞測定・解析技術の臨床応用への期待)
小泉史明, 澤田武志.
細胞, 48(7): 322-326, July 2016

 

泌尿器科癌領域におけるCTC(末梢循環癌細胞)検査
直江道夫, 下山英明, 中里武彦, 長谷部友紀, 辻山和子, 小川良雄, 石毛真行, 松崎正晴, 大澤宏典.
細胞, 48(5): 212-217, May 2016

 

マイクロチップ型セルソーターを用いた血中循環腫瘍細胞の高感度検出・単離方法
渡辺 勝, 洪 泰浩, 山本 信之
Journal of Japanese Biochemical Society, 88(2): 265-269, April 2016

 

Sensitive cytometry based system for enumeration, capture and analysis of gene mutations of circulating tumor cells
Takeshi Sawada, Masaru Watanabe, Yuu Fujimura, Shigehiro Yagishita, Tatsu Shimoyama, Yoshiharu Maeda, Shintaro Kanda, Mayu Yunokawa, Kenji Tamura, Tomohide Tamura, Hironobu Minami, Yasuhiro Koh and Fumiaki Koizumi.
Cancer Science, 107(3): 307-14, March 2016.

 

無薗ソーティングを実現する使い捨て交換型流路チッブを用いるセルソーター(On-chip Sort)
武田一男.
Bio Industry, 33(2): 34-42, February 2016.

 

A novel flow cytometry-based cell capture platform for the detection, capture and molecular characterization of rare tumor cells in blood
Masaru Watanabe, Masakuni Serizawa, Takeshi Sawada, KazuoTakeda, Toshiaki Takahashi, Nobuyuki Yamamoto, Fumiaki Koizumi and Yasuhiro Koh.
Journal of Translational Medicine, 12(1): 143, May 2014.

 

Multicolor Detection of Rare Tumor Cells in Blood Using a Novel Flow Cytometry-Based System
Masaru Watanabe, Yuri Uehara, Namiko Yamashita, Yuu Fujimura, Kaori Nishio, Takeshi Sawada, Kazuo Takeda, Fumiaki Koizumi, Yasuhiro Koh.
Cytometry Part A, 85(3): 206-213, March 2014.

 

Multiparameter Analysis of Apoptosis Using Lab-on-a-Chip Flow Cytometry
Donald Wlodkowic, Joanna Skommer, Jin Akagi, Yuu Fujimura, Kazuo Takeda.
Current Protocols in Cytometry, 66(1), Unit 9.42, October 2013.

 

Kinetic viability assays using DRAQ7 probe
Donald Wlodkowic, Jin Akagi, Jurek Dobrucki, Rachel Errington, Paul J Smith, Kazuo Takeda and Zbigniew Darzynkiewicz.
Current Protocols in Cytometry, Chapter 9: Unit 9.41, July 2013.

 

Real-time cell viability assays using a new anthracycline derivative DRAQ7®
Jin Akagi, Magdalena Kordon, Hong Zhao, Anna Matuszek, Jurek Dobrucki, Rachel Errington, Paul J Smith,Kazuo Takeda, Zbigniew Darzynkiewicz, and Donald Wlodkowic.
Cytometry Part A, 83(2): 227-234, February 2013.

 

Multiparameter Lab-on-a-Chip flow cytometry of the cell cycle.
Joanna Skommer, Jin Akagi, KazuoTakeda, Yuu Fujimura, Khashayar Khoshmanesh, Donald Wlodkowic.
Biosensors and Bioelectronics, 42:586-91, April 2013.

 

Microflow Cytometry in Studies of Programmed Tumour Cell Death
Jin Akagi, Kazuo Takeda, Yuu Fujimura, Anna Matuszek, Khashayar Khoshmanesh, Donald Wlodkowic.
Procedia Engineering, 47: 88-91, February 2012.

 

Establishment of novel detection system for embryonic stem cell-derived hepatocyte-like cells based on nongenetic manipulation with indocyanine green.
Susumu Yoshie, Jun Ito, Sakiko Shirasawa, Tadayuki Yokoyama, Yuu Fujimura, Kazuo Takeda, Masahiro Mizuguchi, Ken Matsumoto, Daihachiro Tomotsune, and Katsunori Sasaki.
Tissue Engineering Part C Methods, 18(1): 12-20, January 2012.

 

Implication of the NorB Efflux Pump in the Adaptation of Staphylococcus aureus to Growth at Acid pH and in Resistance to Moxifloxacin
Que Chi Truong-Bolduc, Gilles R. Bolduc, Ryo Okumura, Brian Celino, Jennifer Bevis, Chun-Hsing Liao, and David C. Hooper.
Antimicrobial Agents and Chemotherapy, 55(7), 3214–3219, July 2011.

 

Small-scale cell analysis by the microfluidic flow cytometer, FISHMAN-R, using a disposable chip.
Fumie Jimma, Yuu Fujimura and Kazuo Takeda.
Cytometry Research, 21(1):31-36, March 2011.

 

Development of a new compact cytometer using a disposable microfluidic chip for contamination-free and biosafety measurement
Kazuo Takeda, Yu Fujimura and Fumie Jimma.
Cytometry Part A, 79(2): 107-117, February 2011.

 

Enumeration, characterization, and collection of intact circulating tumor cells by cross contamination-free flow cytometry;
Masashi Takao and Kazuo Takeda.
Cytometry Part A, 79(2): 93-4, February 2011

 

Development of Defective and Persistent Sendai Virus Vector: a Unique Gene Delivery/Expression System Ideal for Cell Reprogramming;
Ken Nishimura, Masayuki Sano, Manami Ohtaka, Birei Furuta, Yoko Umemura, Yoshiro Nakajima, Yuzuru Ikehara, Toshihiro Kobayashi, Hiroaki Segawa, Satoko Takayasu, Hideyuki Sato, Kaori Motomura, Eriko Uchida, Toshie Kanayasu-Toyoda, Makoto Asashima, Hiromitsu Nakauchi, Teruhide Yamaguchi and Mahito Nakanishi.
Journal of Biological Chemistry, 286(6): 4760-4771, February 2011.

 

Maintenance free biosafety flow cytometer using disposable microfluidic chip (FISHMAN-R)
Kazuo Takeda and Fumie Jimma.
Cytometry Part B, 76B: 405-406, October 2009.

 

Conference Presentations

Rescue of Osteonecrosis by Transplantation of Intact Blood Vessel Stem Cell Niches
Liming Zhao,Malachia Hoover, Matthew Murphy, Lauren Koepke, Michael Lopez, Thomas Ambrosi, Owen Marecic, Holly Steininger, Nestor Deleon, Patricia Nguyen, Michael Longaker and Charles Chan.
ISSCR2019, Los Angeles, CA, USA, June 26-29, 2019.

 

A Method to Isolate and Transplant Mouse Hematopoietic Stem Cells along with their Niches Associated with Improved Functional Hematopoietic Engraftment without Myeloablation
Malachia Hoover, Mimi Borrelli, Thomas Ambrosi, Jin Akagi, Michael Lopez, Jan Sokolo, Gunsagar Gulati, Jan Sokolo, Rahul Sinha, Stephanie Conley, Michael Longaker, Irv Weissman, Aron Newman and Charles Chan.
ISSCR2019, T2113, Los Angeles, CA, USA, June 26-29, 2019.

 

A Novel Quality Control Method for Removal and Monitoring of Residual Pluripotent Stem Cells within a Large Number of Differentiated Cells by Multi-Step Negative Sorting
Kazuo Takeda, Elisa Lam, Fumiyuki Nakagawa, Jin Akagi, Shinichiro Chuma, Takahide Ino and Masayuki Ishige.
ISSCR2019, T3227, Los Angeles, CA, USA, June 26-29, 2019.

 

High-purity isolation method of rare cells for molecular analyses using microfluidic chip type cell sorter
Yasuhiro Ko1, Mio Ikeda, ShunsukeTeraoka, Masayuki Ishige, YuuFujimura, Kazuo Takeda, Jun Oyanagi, Nahomi Tokudome, Yuichi Ozawa, Hiroki Ueda, Nobuyuki Yamamoto.
AACR2019, 2223, Atlanta, GA, USA, March 29-April 3, 2019.

 

A Method to Isolate and Transplant Mouse Hematopoietic Stem Cells along with their Niches Allowing Functional Hematopoietic Stem Cell Engraftment without Myeloablation
Mimi Borrelli, Michael Lopez, Gunsagar Gulati, Jan Sokol, Jin Akagi, Rahul Sinha, Rachel Brewer, Stephanie Conley, Aron Newman, Irv Weissman, Michael Longaker and Charles Chan.
ISSCR2018, W1100, Melbourne, Australia, June 20-23, 2018.

 

Multiparametric Cell Sorting with Minimal Impact on Cell Viability or Function
Peter Lopez and Jin Akagi.
CYTO2018, 79, Prague, Czech Republic, April 28-May 2, 2018.

 

Characterization of Exosomes from Cancer Using Surface Marker Expression, Exosome Sorting, and MicroRNA Profiling
Steven McClellan, Mary Patton, Girijesh Patel, Jin Akagi and Ajay Singh.
CYTO2018, 74, Prague, Czech Republic, April 28-May 2, 2018.

 

Development of a Cancer Stem Cell 3D Spheroid Assay System for HTS Image-Based Screening
Steven McClellan, Antonio Ward, Adam Keeton, Christian Manganti, Jin Akagi, Yuu Fujimura and Gary Piazza.
CYTO2018, B183, Prague, Czech Republic, April 28-May 2, 2018.

 

Development of Single-Cell Expression Analysis System for Circulating Tumor Cells
Yuu Fujimura, Masayuki Ishige, Jin Akagi, Kosuke Osawa, Takahide Ino and Kazuo Takeda.
CYTO2017, B39, Boston, Massachusetts, USA, June 10–14, 2017.

 

Damage-Free Sorting Technique Using Disposable Microfluidic Chip for Analysis of Intracellular Interactions
Jin Akagi, Masayuki Ishige, Yuji Morishita, Yohsuke Bansho, Takahide Ino, Yuu Fujimura and Kazuo Takeda.
CYTO2017, B32, Boston, Massachusetts, USA, June 10–14, 2017.

 

The Emulsion Sorting Technology Platform for High-Throughput Screening Assays in Various Fields of Biology
Yohsuke Bansho, Jin Akagi, Takahide Ino, Kosuke Osawa, Tomiko Tanaka, Masayuki Ishige, Yuu Fujimura and Kazuo Takeda.
CYTO2016, B138, Seattle, Washington, USA, June 11–15, 2016.

 

Water-in-Oil Emulsion Sorting for Digital PCR and Single Cell Analysis Using Air Pressure Pulse Flow in Disposable Microfluidic Chip
Jin Akagi, Yuu Fujimura, Yoshie Kawase and Kazuo Takeda.
CYTO2015, B235, Glasgow, Scotland, June 26–30, 2015.

 

Negative Cell Sorting for Removal of Undifferentiated Cells for Regenerative Medicine
Takahide Ino, Haixin Zhang, Masayuki Ishige, Kazuhiro Aiba, Yuu Fujimura, Norio Nakatsuji and Kazuo Takeda.
CYTO2015, B242, Glasgow, Scotland, June 26–30, 2015.

 

A novel flow cytometry-based cell capture platform for the detection, capture and molecular characterization of circulating tumor cells
Yasuhiro Koh, Masaru Watanabe, Takeshi Sawada, Hirotsugu Kenmotsu, Masakuni Serizawa, Masayuki Ishige, Kazuo Takeda, Nobuyuki Yamamoto, Toshiaki Takahashi and Fumiaki Koizumi.
AACR2014, San Diego, CA, USA, April 5-9, 2014.

 

Clinical feasibility study of a novel cytometry-based-system for the detection of circulating tumor cells (CTCs) of patients with lung cancer
Takeshi Sawada, Yukiko Ito, Masaru Watanabe, Yuu Fujimura, Shinji Nakamichi, Shintaro Kanda, Hidehito Horinouchi, Yutaka Fujiwara, Hiroshi Nokihara, Noboru Yamamoto, Tomohide Tamura, Yasuhiro Koh and Fumiaki Koizumi.
AACR2013, #1445, Washington, DC, USA, April 6-10, 2013.

 

Molecular characterization of captured circulating tumor cells (CTCs) with a novel cell sorting system
Masaru Watanabe, Takeshi Sawada, Masakuni Serizawa, Yukiko Ito, Yuu Fujimura, Fumiaki Koizumi and Yasuhiro Koh.
AACR2013, #5105, Washington, DC, USA, April 6-10, 2013.

 

Multivariate analysis of apoptotic markers versus cell cycle phase in living human cancer cells by microfluidic cytometry
Jin Akagi, Joanna Skommer, Anna Matuszek, Kazuo Takeda, Yuu Fujimura, Khashayar Khoshmanesh, Kourosh Kalantar-Zadeh, Arnan Mitchell, Rachel Errington,Paul J Smith, Zbigniew Darzynkiewicz and Donald Wlodkowic.
SPIE Microfluidics, BioMEMS, and Medical Microsystems XI, San Francisco, CA, USA, February 2, 2013.

 

Detection of circulating tumor cells for malignant epithelial tumor using a novel cytometry-based system
Yukiko Ito, Takeshi Sawada, Masaru Watanabe, Yuu Fujimura, Jun Hashimoto, Makoto Kodaira, Mayu Yunokawa, Harukaze Yamamoto, Kan Yonamori, Chikako Shimizu, Kenji Tamura, Yasuhiro Fujiwara, Yasuhiro Koh and Fumiaki Koizumi.
AACR2013, #1446, Washington, DC, USA, April 6-10, 2013.
使い捨てマイクロ流路チップ上でのパルス流による無菌的ダメージレスセルソーター
藤村祐, 武田一男.
第11回日本再生医療学会総会, 横浜, June 2012.

 

ICG(インドシアニングリーン)を用いた新規肝細胞検出・分離システムの開発
吉江進, 増田章子, 伊藤潤, 滝澤佐季子, 永井美圭, 横山忠幸, 藤村裕, 武田一男, 水口昌宏, 松本健, 岳鳳鳴, 友常大八郎, 佐々木克典.
第11回 日本再生医療学会総会, 横浜, June 2012.

 

Evaluation of a novel flow cytometry-based system for the detection of circulating tumor cells (CTCs)
Masaru Watanabe, Yuri Uehara, Namiko Yamashita, Yuu Fujimura, Kaori Nishio, Junko Suzuki, Fumiaki Koizumi and Yasuhiro Koh.
AACR2012, #1735, Chicago, IL, USA, March 31-April 4, 2012.

 

アプタマー修飾磁性ビーズを用いたアミロイドβオリゴマーのフローサイトメトリー検出
塚越かおり, 阿部公一, 武田一男, 藤村祐, 早出広司, 池袋一典.
化学会第9 92春季年会, 慶応大日吉, March 2012.

 

歯周病関連細菌に対するシタフロキサシンのin vitoro, 抗菌活性の検討:新鮮臨床分離株に対する公開ん活性および殺菌力
難波栄子, 千葉めぐみ, 奥村亮, 神田裕子, 星野一樹, 金子明寛.
第31回日本歯科薬物療法学会, 幕張, July 2011.

 

使い捨てマイクロ流路チップ上でのパルス流によるダメージレスセルソーターの開発
藤村祐, 武田一男.
第21回日本サイトメトリー学会学術集会, 京都, June 2011.

 

多検体対応の使い捨てマルチマイクロ流路チップを用いたフローサイトメーターの開発(FISHMAN-Multi)
武田一男, 藤村 祐.
化学とマイクロ・ナノシステム研究会, June 10, 2011.

 

Development of disposable microfluidic chip based cell sorter for high cell purification with no damage
Yuu Fujimura and Kazuo Takeda.
CYTO2011, B144, Baltimore, MD, USA, May 21-25, 2011.

 

Cell clump analysis for flow cytometric enumeration of circular tumor cells by using disposable microfluidic chip
Kazuo Takeda and Namiko Yamashita.
CYTO2011, B143, Baltimore, MD, USA, May 21-25, 2011.

 

ICG(インドシアニングリーン)を用いた新規肝細胞検出・分離システムの開発
吉江進, 伊藤潤, 白澤佐季子, 横山忠幸, 藤村祐, 武田一男, 友常大八郎, 佐々木克典.
第10回日本再生医療学会総会, 東京, March 2011.

 

Expression of the Staphylococcus aureus Efflux Pumps NorA, B, and C in Response to Acidification
Que Chi Truong-Bolduc, Gilles Bolduc, Brian Celino, Jennifer Bevis, Y. Ding, Regis Villet and David Hooper.
ICAAC2010, Boston, MA, USA, September 12-15, 2010.

 

細胞表面マーカーによるイヌ末梢血好塩基球同定方法の試み
大森啓太郎, 神間史恵, 武田一男, 松田浩珍, 田中あかね.
第150回日本獣医学会学術集会, 帯広, September 2010.

 

使い捨てチップ型マイクロ流路フローセルによるフローサイトメータの性能向上技術とその進化系としての多検体対応技術
武田一男, 神間史恵.
第20回日本サイトメトリー学会学術集会, 東京, June 2010.

 

使い捨てチップ型マイクロ流路フローセルによるフローサイトメータが可能とする細胞解析のアプリケーション
神間史恵, 武田一男.
第20回日本サイトメトリー学会学術講演会, 東京, June 2010.

 

ディスポーザブルチップ搭載フローサイトメーターを用いたCTC計数と解析の新展開
高尾雅, 神間史恵, 武田一男, 小泉史明.
第20回日本サイトメトリー学会学術集会, 東京, June 2010.

 

Development of cross-contamination free flow cytometer using a disposable multi flow channel chip with side scattered light detection optics.
Kazuo Takeda and Fumie Jimma.
CYTO2010, P227, Seattle, WA, USA, May 8-12, 2010.

 

Enumeration and characterization of circulating tumor cells with cross-contamination free flow cytometer
Masashi Takao, Fumie Jimma and Kazuo Takeda.
CYTO2010, P112, Seattle, WA, USA, May 8-12, 2010.

 

使い捨て型マイクロ流路チップを用いた超小型フローサイトメーター(FISHMAN-R)が可能とする微量血球分析と細菌分析
武田一男, 神間史恵.
第20回化学とマイクロ・ナノシステム研究会, 金沢, November 2009.

 

Maintenance free biosafety flow cytometer using disposable microfluidic chip (FISHMAN-R)
Kazuo Takeda and Fumie Jimma.
Clinical Cytometry 2009, Jacksonville, FL, USA, 2009

 

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