• On-chip Sort

    The world's first cell sorter using disposable microfluidic chip for
    damage-free and sterile sorting

    PLAY MOVIE
  • On-chip Sort

    The world's first cell sorter using disposable microfluidic chip for
    damage-free and sterile sorting

    PLAY MOVIE
  • On-chip Sort

    The world's first cell sorter using disposable microfluidic chip for
    damage-free and sterile sorting

    PLAY MOVIE
  • On-chip Sort

    The world's first cell sorter using disposable microfluidic chip for
    damage-free and sterile sorting

    PLAY MOVIE
  • On-chip Sort

    The world's first cell sorter using disposable microfluidic chip for
    damage-free and sterile sorting

    PLAY MOVIE

APPLICATIONS

A few of our unique applications using On-chip Sort

ADVANTAGES

Some benefits in using the world's first microfluidic cell sorter

PRODUCTS

On-chip Biotechnologies' product lineup

SORTING TECHNOLOGY

Our unique sorting technique explained

NEWS/EVENTS

News and upcoming events

COMPANY

About our company

COME FOR A QUICK ON-CHIP VIDEO TOUR

Find out more about our gentle cell sorter in our quick video.

PLAY MOVIE

01

APPLICATIONS

A few of our unique applications using On-chip Sort. We have users in various fields of research.

EXTENDING YOUR RESEARCH WITH ON-CHIP SORT

This microfluidic chip cell sorter has allowed users to do what they could not do before. Now you have a tool to make your brilliant ideas come to life!

STEM CELL RESEARCH

iPSCs, iPSC-derived cells and stem cells can be sorted without damaging the cells. Sorting in aseptic condition while preventing cross-contamination will greatly benefit research in cell transplantation.

CELL CLUSTERS & ORGANOIDS

Cell clusters, such as spheroids and organoids of diameter up to 150μm can be sorted while keeping the shape intact. Primary cell clumps and small organisms are also be sorted.

RARE CELL SORTING (CTC)

Rare cells (<0.01%) can be collected efficiently using our multi-step sorting technique. For example, Circulating Tumor Cells (CTCs) can be collected efficiently in an hour after sample pre-treatment.

EMULSION DROPLETS

Water-in-oil emulsion droplets can be sorted using oil as sheath fluid. Those droplets are used as a way to compartmentalize a sample, and reactions and cultivation of microbes ca be carried out in each individual droplet.

CELL CULTURING

Fragile cells sorted using conventional method show slow or no growth due to sorting damage. Improved cell viability and growth rate are observed for cells collected using our gentle sorting techniques.

ENVIRONMENTAL MICROORGANISMS

Microorganisms, such as soil microbes and marine organisms, are often susceptible to the sorting conditions. On-chip Sort allows for the use of any liquid as sheath fluid.

Neurons Sperms cells Cardiomyocytes
Phytoplanktons Plant cells Hepatocytes
Yeasts Gel balls Pollen
02

ADVANTAGES

The use of the disposable microfluidic chip as the core of On-chip Sort realizes great benefits over conventional cell sorters. Some of many advantages of On-chip Sort are listed below:

DAMAGE-FREE

Our unique “flow-shift” sorting technique allows for damage-free cell sorting.

CONTAMINATION-FREE

Exchangeable fluidics mean there is no sample carry-over or sample coming in contact with the instrument.

MEDIUM OF CHOICE

Wide range of medium can be used as sorting media to fit sample requirement.

EASY OPERATION

No calibration means sorting is only a few steps away. No washing either!

SMALL FOOTPRINT

It is small enough to fit well inside a biosafety cabinet for aseptic sorting.

LOW STARTING VOLUME

Sample can be as low as 20μL. There is also no dead volume.

Comparison with conventional cell sorter

Conventional On-chip Sort
Cell damage Possibility of damage Damage-free
Contamination Microbial contamination may occur Sterile and contamination-free
Sheath fluid Fixed Choose from a variety of media
03

PRODUCT

On-chip Biotechnologies currently offer two instruments: On-chip Sort (cell sorter) and On-chip SPiS (single cell dispenser)

Microfluidic chip cell sorter
“ON-CHIP SORT”

Revolutionary cell sorter using disposable microfluidic chip for damage-free and sterile sorting.

Single cell plate dispenser
“ON-CHIP SPiS”

Cell dispenser for accurate and gentle single cell plating into 96 or 384 well plate.

04

SORTING TECHNOLOGY

Jet-in-air or Cuvette hybrid cell sorters have multiple processes that triggers sorter-induced cellular stress (SICS). On-chip Sort allows for much gentler sorting of cells compared to conventional sorters because the use of microfluidic chip has eliminated such damaging steps.

Conventional Sorter On-chip Sort
Sorting Pressure Approx. 40psi Approx. 0.3psi
High-speed Collision Cells collide at high-speed when collected No collision
Electrostatic Charging Cells are stressed due to charging No electrostatic charging
Aerosol Generation Potentially hazardous for users No aerosols
Sorting Medium Must use specified sorting medium Most standard media accepted
Washing Post-sort wash with harsh chemicals No post-sort washing
05

NEWS/EVENT

Latest information on updates, upcoming events and press-releases

06

COMPANY

Here are some details of the company

Headquarters On-chip Biotechnologies Co., Ltd
President CEO Masayuki Kobayashi
Address 203 Venture Port, 2-24-16, Naka-cho, Koganei-shi, Tokyo, 184-0012, Japan
Phone/FAX Phone: +81-42-385-0461  FAX: +81-42-385-0462
E-mail info@on-chip.co.jp
Capital JPY100,000,000
Established April 1st 2005
Number of Staffs 20 staffs including 8 Doctorates (as of April 2020)
Board members CEO Masayuki Kobayashi, CTO Kazuo Takeda