On-chip Droplet Generator allows mass production of micrometer-sized water-in-oil (W/O) emulsion droplets and gel microdrops (GMDs) with a unique microfluidic chip. The individual droplets function as miniaturized vessels for reactions such as microbial/cell cultivation and genetic analysis. Droplet technology is now recognized as a prominent high-throughput screening method allowing single cell analysis or simultaneous assay of more than 1 million samples.
FEATURES
Generation of stable droplets-
On-chip Droplet Generator allows production of stable and monodisperse droplets.
Structure of microchannel 
Easy adjustment of droplet size-
Droplet size can be easily adjusted between 60 µm and 220 µm in diameter by regulating the pressure applied to the sample and oil reservoirs.
See “Reagents/Chips” page for details for each microfluidic chip used for Droplet Generator
Real-time monitoring of droplet generation-
Droplet size can be viewed and monitored real-time with the supplied monitoring device.
Types of droplets generated
On-chip Droplet Generator can produce two types of droplets: water-in-oil (W/O) emulsion droplets and gel microdrops (GMDs).
W/O droplet |
GMD |
|
|---|---|---|
| No. of droplets | ≥ 1 million droplets | ≥ 1 million droplets |
| Contents | Aqueous liquid (medium, etc.) | Solid (agarose, gelatin, etc.) |
| Transfer of substances | Maintained inside (depending on polarity) | Extracellular fluid including reagent infiltrates the inside |
| Usage | Environmental microbial screening, etc. | Variant screening, etc. |
| Immediately after encapsulation After cultivation |
E. coli |
Lung cancer-derived cell line |
Utilization of On-chip Droplet Generator and On-chip Sort
On-chip Sort utilizes microfluidic chip technology that allows oil to be used as sheath fluid, which makes the selection of W/O droplets with microorganisms possible.
See also “On-chip Sort” page for details.
On-chip Droplet Selector is used in various research areas.
See also “Application|Droplet” for details of droplet technology.
SPEC & Product Information
Main unit size
| Size | 270 (W) x 270 (D) x 280 (H) mm |
|---|---|
| Weight | 10kg |
Main unit
| Liquid delivery pressure | 0.1 – 80 kPa |
|---|---|
| Maximum liquid delivery line | 3 lines (sample, oil, other reagents) |
| Droplet size generated | Diameter 60 – 220 μm (can be adjusted discretionally) |
| Generation rate | Approx. 100,000 droplets/3 min (for generation of 100 μm droplets) |
| Regulation | Laptop PC (Windows 10) |
| Power input | AC 100-240V, 50/60Hz |
| Power consumption | 1.2A typ (ACIN 100V) |
Monitoring unit
| Size | 215 (W) x 310 (D) x 430 (H) mm |
|---|---|
| Accessories | Monitor camera, Peltier unit, Controller |
| Temperature range | ー5 ～ 70℃ |
| Ambient environment | Temperature 5 – 40˚C, humidity ≤ 85% RH |
| Power input | DC24 V |
| Power consumption | 6.5 A max |
On-chip Droplet Generator Product List
| Product Number | Product Name | Specifications |
|---|---|---|
| 60003 | On-chip Droplet Generator (Monitoring unit with temperature control) | Main unit, chip holder, monitoring unit with temperature control |
| 60002-b | On-chip Droplet Generator (EU Model) | Instrument only Monitoring unit without temperature control & DG1 Chip holder |
Product model varies depending on the region. Please contact us for details.
See also “Reagents/Chips” page for details of reagents/microfluidic chips used for Droplet Generator.