Cell
Design and validation of functionalized redox-responsive hydrogel beads for high-throughput screening of antibody-secreting mammalian cells.
Diah Anggraini Wulandari, Kyosuke Tsuru, Kosuke Minamihata, Rie Wakabayashi, Go Egami, Yoshinori Kawabe, Masamichi Kamihira, Masahiro Goto, Noriho Kamiya.
Journal of Bioscience and Bioengineering, 138 (1) :89–95, Jul. 2024.
Claudin 18.2 Inhibits the Proliferation of Small-Cell Lung Cancer Cells via G0/G1 Cell-Cycle Arrest.
Korehito Kashiwagi, Tadasuke Miyazawa, Hanako Sato-Yazawa, Jun Ishii, Kakeru Kohno, Koudai Konno, Chie Miyata-Hiramatsu, Tamotsu Kamimura, Shunsuke Miyata, Takuya Yazawa.
Dokkyo Medical Journal 3(1): 16-23, Mar. 2024.
A Functional Hydrogel Bead-Based High-Throughput Screening System for Mammalian Cells with Enhanced Secretion of Therapeutic Antibodies.
Diah Anggraini Wulandari, Kyosuke Tsuru, Kosuke Minamihata, Rie Wakabayashi, Masahiro Goto, Noriho Kamiya.
ACS Biomaterials Science & Engineering, 10 (1) :628–36, Jan. 2024.
Large-scale single-cell cloning of genome-edited cultured human cells by On-chip SPiS.
Gou Takahashi, Yuichiro Miyaoka.
STAR Protocols, 4 (3) :102364, Sep. 2023.
Oxygen–Glucose Deprived Peripheral Blood Mononuclear Cells Protect Against Ischemic Stroke.
Yutaka Otsu, Masahiro Hatakeyama, Takeshi Kanayama, Natsuki Akiyama, Itaru Ninomiya, Kaoru Omae, Taisuke Kato, Osamu Onodera, Masanori Fukushima, Takayoshi Shimohata, Masato Kanazawa.
Neurotherapeutics, 20 (5) :1369–87, Sep. 2023.
Suspendable Hydrogel Nanovials for Massively Parallel Single-Cell Functional Analysis and Sorting
Joseph de Rutte, Robert Dimatteo, Maani M. Archang, Mark van Zee, Doyeon Koo, Sohyung Lee, Allison C. Sharrow, Patrick J. Krohl, Michael Mellody, Sheldon Zhu, James V. Eichenbaum, Monika Kizerwetter, Shreya Udani, Kyung Ha, Richard C. Willson, Andrea L. Bertozzi, Jamie B. Spangler, Robert Damoiseaux, and Dino Di Carlo.
ACS Nano, 16(5):7242-7257, March 2022.
High-purity Isolation for Genotyping Rare Cancer Cells from Blood Using a Microfluidic Chip Cell Sorter
MIO IKEDA, YASUHIRO KOH, JUN OYANAGI, SHUNSUKE TERAOKA, MASAYUKI ISHIGE, YUU FUJIMURA, KAZUO TAKEDA, NAHOMI TOKUDOME, YUICHI OZAWA, HIROKI UEDA and NOBUYUKI YAMAMOTO.
Anticancer Research, 42(1):407-417, January 2022.
Sorting single-cell microcarriers using commercial flow cytometers
Joseph de Rutte, Robert Dimatteo, Sheldon Zhu, Maani M. Archang, and Dino Di Carlo.
SLAS Technology, 27(2):150–59, October 2021.
Hedgehog interacting protein–expressing lung fibroblasts suppress lymphocytic inflammation in mice
Jeong H. Yun, ChangHee Lee, Tao Liu, Siqi Liu, Edy Y. Kim, Shuang Xu, Jeffrey L. Curtis, Luca Pinello, Russell P. Bowler, Edwin K. Silverman, Craig P. Hersh, and Xiaobo Zhou.
JCI Insight, 6(17), August 2021.
Microfluidics sorting enables the isolation of an intact cellular pair complex of CD8+ T cells and antigen-presenting cells in a cognate antigen recognition-dependent manner
Soichiro Kuwabara, Yoshihiko Tanimoto, Mie Okutani, Meng Jie, Yasunari Haseda, Yumi Kinugasa-Katayama, Taiki Aoshi.
PLOS ONE, 16(6):e0252666, June 2021.
Upregulation of glucocorticoid receptor-mediated glucose transporter 4 in enzalutamide-resistant prostate cancer
Seiji Hoshi, Satoru Meguro, Hitomi Imai, Yuta Matsuoka, Yuki Yoshida, Akihumi Onagi, Ryo Tanji, Ruriko Honda-Takinami, Kanako Matsuoka, Tomoyuki Koguchi, Junya Hata, Yuichi Sato, Hidenori Akaihata, Masao Kataoka, Soichiro Ogawa, and Yoshiyuki Kojima.
Cancer Science, 112(5):1899–1910, February 2021.
Regulation of MRE11A by UBQLN4 leads to cisplatin resistance in patients with esophageal squamous cell carcinoma
Tomohiro Murakami, Yoshiaki Shoji, Tomohiko Nishi, Shu Ching Chang, Ron D. Jachimowicz, Sojun Hoshimoto, Shigeshi Ono, Yosef Shiloh, Hiroya Takeuchi, Yuko Kitagawa, Dave S. B. Hoon, and Matias A. Bustos.
Molecular Oncology, 15(4):1069–87, February 2021.
Establishment of a novel method for the production of chimeric mouse embryos using water-in-oil droplets
Hiroyuki Imai, Soichiro Tsuda, Tokuko Iwamori, Kiyoshi Kano, Ken Takeshi Kusakabe, Etsuro Ono
Experimental Animals, 70(1), 84–90, 2021.
A single-cell transcriptomic and anatomic atlas of mouse dorsal raphe Pet1 neurons
Benjamin W Okaty, Nikita Sturrock, Yasmin Escobedo Lozoya, YoonJeung Chang, Rebecca A Senft, Krissy A Lyon, Olga V Alekseyenko, and Susan M Dymecki.
eLife, 9: e55523, June 2020.
Successful selection of mouse sperm with high viability and fertility using microfluidics chip cell sorter
Satohiro Nakao, Toru Takeo, Hitomi Watanabe, Gen Kondoh and Naomi Nakagata.
Scientific Reports, 10: 8862, June 2020.
Establishing typical values for hemocyte mortality in individual California mussels, Mytilus californianus
Nicole E Moyen, Paul A Bump, George N Somero, and Mark W Denny.
Fish and Shellfish Immunology, 100: 70-79, May 2020.
Antiparkinson Drug Benztropine Suppresses Tumor Growth, Circulating Tumor Cells, and Metastasis by Acting on SLC6A3/DAT and Reducing STAT3
Chiharu Sogawa, Takanori Eguchi, Manh Tien Tran, Masayuki Ishige, Kilian Trin, Yuka Okusha, Eman Ahmed Taha, Yanyin Lu, Hotaka Kawai, Norio Sogawa, Masaharu Takigawa, Stuart K. Calderwood, Kuniaki Okamoto, and Ken Ichi Kozaki.
Cancers, 12(2): 523, February 2020.
Spindle cell renal cell carcinoma diagnosed after sunitinib treatment for chromophobe renal cell carcinoma
Ruriko Honda‐Takinami, Kei Ishibashi, Akifumi Onagi, Ryo Tanji, Kanako Matsuoka, Seiji Hoshi, Tomoyuki Koguchi, Junya Hata, Michihiro Yabe, Yuichi Sato, Hidenori Akaihata, Masao Kataoka, Soichiro Ogawa, Nobuhiro Haga, and Yoshiyuki Kojima.
IJU Case Reports, 3(2):36–39, January 2020.
Detection of circulating sarcoma tumor cells using a microfluidic chip-type cellsorter
Nobuhiko Hasegawa, Ikuko Takeda Nakamura, Toshihide Ueno, Shinya Kojima, Masahito Kawazu, Keisuke Akaike, Taketo Okubo, Tatsuya Takagi, Yoshiyuki Suehara, Takuo Hayashi, Tsuyoshi Saito, Kazuo Kaneko, Hiroyuki Mano, and Shinji Kohsaka.
Scientific Reports, 9: 20047, December 2019.
A novel therapeutic approach using peripheral blood mononuclear cells preconditioned by oxygen-glucose deprivation
Masahiro Hatakeyama, Masato Kanazawa, Itaru Ninomiya, Kaoru Omae, Yasuko Kimura, Tetsuya Takahashi, Osamu Onodera, Masanori Fukushima, and Takayoshi Shimohata.
Scientific Reports, 9(1):1–14, November 2019.
Development of a Highly Sensitive Technique for Capturing Renal Cell Cancer Circulating Tumor Cells
Michio Naoe, Chiho Kusaka, Mika Ohta, Yuki Hasebe, Tsutomu Unoki, Hideaki Shimoyama, Takehiko Nakasato, Kazuhiko Oshinomi, Jun Morita, Kohzo Fuji, Yoshio Ogawa, Mana Tsukada, Masataka Sunagawa, and Hikaru Ishii.
Diagnostics, 96(3), August 2019.
Isolation and molecular analysis of circulating tumor cells from lung cancer patients using a microfluidic chip type cell sorter
Masaru Watanabe, Hirotsugu Kenmotsu, Ryo Ko, Kazushige Wakuda, Akira Ono, Hisao Imai, Tetsuhiko Taira, Tateaki Naito, Haruyasu Murakami, Masato Abe, Masahiro Endo, Takashi Nakajima, Toshiaki Takahashi, and Yasuhiro Koh.
Cancer Science, 109(8), July 2018.
Ecel1 knockdown with an AAV2-mediated CRISPR/Cas9 system promotes optic nerve damage-induced RGC death in the mouse retina
Kota Sato, Yukihiro Shiga, Yurika Nakagawa, Kosuke Fujita, Koji M. Nishiguchi, Hiroshi Tawarayama, Namie Murayama, Shigeto Maekawa, Takeshi Yabana, Kazuko Omodaka, Shota Katayama, Qiwei Feng, Satoru Tsuda, and Toru Nakazawa.
Investigative Ophthalmology & Visual Science, 59(10), July 2018.
Nascent Induced Pluripotent Stem Cells Efficiently Generate Entirely iPSC-Derived Mice while Expressing Differentiation-Associated Genes
Bhishma Amlani, Yiyuan Liu, Taotao Chen, Ly-Sha Ee, Peter Lopez, Adriana Heguy, Effie Apostolou, Sang Yong Kim, and Matthias Stadtfeld.
Cell Report, 22(4): 876–884, January 2018.
Microproteomics with microfluidic-based cell sorting: Application to 1000 and 100 immune cells
Kie Kasuga, Yasutake Katoh, Keisuke Nagase, and Kazuhiko Igarashi.
Proteomics, 17(13-14): 1600420, July 2017.
Sensitive cytometry based system for enumeration, capture and analysis of gene mutations of circulating tumor cells
Takeshi Sawada, Masaru Watanabe, Yuu Fujimura, Shigehiro Yagishita, Tatsu Shimoyama, Yoshiharu Maeda, Shintaro Kanda, Mayu Yunokawa, Kenji Tamura, Tomohide Tamura, Hironobu Minami, Yasuhiro Koh and Fumiaki Koizumi.
Cancer Science, 107(3): 307-14, March 2016.
A novel flow cytometry-based cell capture platform for the detection, capture and molecular characterization of rare tumor cells in blood
Masaru Watanabe, Masakuni Serizawa, Takeshi Sawada, KazuoTakeda, Toshiaki Takahashi, Nobuyuki Yamamoto, Fumiaki Koizumi and Yasuhiro Koh.
Journal of Translational Medicine, 12(1): 143, May 2014.
Multicolor Detection of Rare Tumor Cells in Blood Using a Novel Flow Cytometry-Based System
Masaru Watanabe, Yuri Uehara, Namiko Yamashita, Yuu Fujimura, Kaori Nishio, Takeshi Sawada, Kazuo Takeda, Fumiaki Koizumi, Yasuhiro Koh.
Cytometry Part A, 85(3): 206-213, March 2014.
Multiparameter Analysis of Apoptosis Using Lab-on-a-Chip Flow Cytometry
Donald Wlodkowic, Joanna Skommer, Jin Akagi, Yuu Fujimura, Kazuo Takeda.
Current Protocols in Cytometry, 66(1), Unit 9.42, October 2013.
Kinetic viability assays using DRAQ7 probe
Donald Wlodkowic, Jin Akagi, Jurek Dobrucki, Rachel Errington, Paul J Smith, Kazuo Takeda and Zbigniew Darzynkiewicz.
Current Protocols in Cytometry, Chapter 9: Unit 9.41, July 2013.
Real-time cell viability assays using a new anthracycline derivative DRAQ7®
Jin Akagi, Magdalena Kordon, Hong Zhao, Anna Matuszek, Jurek Dobrucki, Rachel Errington, Paul J Smith,Kazuo Takeda, Zbigniew Darzynkiewicz, and Donald Wlodkowic.
Cytometry Part A, 83(2): 227-234, February 2013.
Multiparameter Lab-on-a-Chip flow cytometry of the cell cycle.
Joanna Skommer, Jin Akagi, KazuoTakeda, Yuu Fujimura, Khashayar Khoshmanesh, Donald Wlodkowic.
Biosensors and Bioelectronics, 42:586-91, April 2013.
Microflow Cytometry in Studies of Programmed Tumour Cell Death
Jin Akagi, Kazuo Takeda, Yuu Fujimura, Anna Matuszek, Khashayar Khoshmanesh, Donald Wlodkowic.
Procedia Engineering, 47: 88-91, February 2012.
Establishment of novel detection system for embryonic stem cell-derived hepatocyte-like cells based on nongenetic manipulation with indocyanine green.
Susumu Yoshie, Jun Ito, Sakiko Shirasawa, Tadayuki Yokoyama, Yuu Fujimura, Kazuo Takeda, Masahiro Mizuguchi, Ken Matsumoto, Daihachiro Tomotsune, and Katsunori Sasaki.
Tissue Engineering Part C Methods, 18(1): 12-20, January 2012.
Implication of the NorB Efflux Pump in the Adaptation of Staphylococcus aureus to Growth at Acid pH and in Resistance to Moxifloxacin
Que Chi Truong-Bolduc, Gilles R. Bolduc, Ryo Okumura, Brian Celino, Jennifer Bevis, Chun-Hsing Liao, and David C. Hooper.
Antimicrobial Agents and Chemotherapy, 55(7), 3214–3219, July 2011.
Small-scale cell analysis by the microfluidic flow cytometer, FISHMAN-R, using a disposable chip.
Fumie Jimma, Yuu Fujimura and Kazuo Takeda.
Cytometry Research, 21(1):31-36, March 2011.
Development of a new compact cytometer using a disposable microfluidic chip for contamination-free and biosafety measurement
Kazuo Takeda, Yu Fujimura and Fumie Jimma.
Cytometry Part A, 79(2): 107-117, February 2011.
Enumeration, characterization, and collection of intact circulating tumor cells by cross contamination-free flow cytometry;
Masashi Takao and Kazuo Takeda.
Cytometry Part A, 79(2): 93-4, February 2011
Development of Defective and Persistent Sendai Virus Vector: a Unique Gene Delivery/Expression System Ideal for Cell Reprogramming;
Ken Nishimura, Masayuki Sano, Manami Ohtaka, Birei Furuta, Yoko Umemura, Yoshiro Nakajima, Yuzuru Ikehara, Toshihiro Kobayashi, Hiroaki Segawa, Satoko Takayasu, Hideyuki Sato, Kaori Motomura, Eriko Uchida, Toshie Kanayasu-Toyoda, Makoto Asashima, Hiromitsu Nakauchi, Teruhide Yamaguchi and Mahito Nakanishi.
Journal of Biological Chemistry, 286(6): 4760-4771, February 2011.
Maintenance free biosafety flow cytometer using disposable microfluidic chip (FISHMAN-R)
Kazuo Takeda and Fumie Jimma.
Cytometry Part B, 76B: 405-406, October 2009.
Conference
Rescue of Osteonecrosis by Transplantation of Intact Blood Vessel Stem Cell Niches
Liming Zhao,Malachia Hoover, Matthew Murphy, Lauren Koepke, Michael Lopez, Thomas Ambrosi, Owen Marecic, Holly Steininger, Nestor Deleon, Patricia Nguyen, Michael Longaker and Charles Chan.
ISSCR2019, Los Angeles, CA, USA, June 26-29, 2019.
A Method to Isolate and Transplant Mouse Hematopoietic Stem Cells along with their Niches Associated with Improved Functional Hematopoietic Engraftment without Myeloablation
Malachia Hoover, Mimi Borrelli, Thomas Ambrosi, Jin Akagi, Michael Lopez, Jan Sokolo, Gunsagar Gulati, Jan Sokolo, Rahul Sinha, Stephanie Conley, Michael Longaker, Irv Weissman, Aron Newman and Charles Chan.
ISSCR2019, T2113, Los Angeles, CA, USA, June 26-29, 2019.
A Novel Quality Control Method for Removal and Monitoring of Residual Pluripotent Stem Cells within a Large Number of Differentiated Cells by Multi-Step Negative Sorting
Kazuo Takeda, Elisa Lam, Fumiyuki Nakagawa, Jin Akagi, Shinichiro Chuma, Takahide Ino and Masayuki Ishige.
ISSCR2019, T3227, Los Angeles, CA, USA, June 26-29, 2019.
High-purity isolation method of rare cells for molecular analyses using microfluidic chip type cell sorter
Yasuhiro Ko1, Mio Ikeda, ShunsukeTeraoka, Masayuki Ishige, YuuFujimura, Kazuo Takeda, Jun Oyanagi, Nahomi Tokudome, Yuichi Ozawa, Hiroki Ueda, Nobuyuki Yamamoto.
AACR2019, 2223, Atlanta, GA, USA, March 29-April 3, 2019.
A Method to Isolate and Transplant Mouse Hematopoietic Stem Cells along with their Niches Allowing Functional Hematopoietic Stem Cell Engraftment without Myeloablation
Mimi Borrelli, Michael Lopez, Gunsagar Gulati, Jan Sokol, Jin Akagi, Rahul Sinha, Rachel Brewer, Stephanie Conley, Aron Newman, Irv Weissman, Michael Longaker and Charles Chan.
ISSCR2018, W1100, Melbourne, Australia, June 20-23, 2018.
Multiparametric Cell Sorting with Minimal Impact on Cell Viability or Function
Peter Lopez and Jin Akagi.
CYTO2018, 79, Prague, Czech Republic, April 28-May 2, 2018.
Characterization of Exosomes from Cancer Using Surface Marker Expression, Exosome Sorting, and MicroRNA Profiling
Steven McClellan, Mary Patton, Girijesh Patel, Jin Akagi and Ajay Singh.
CYTO2018, 74, Prague, Czech Republic, April 28-May 2, 2018.
Development of a Cancer Stem Cell 3D Spheroid Assay System for HTS Image-Based Screening
Steven McClellan, Antonio Ward, Adam Keeton, Christian Manganti, Jin Akagi, Yuu Fujimura and Gary Piazza.
CYTO2018, B183, Prague, Czech Republic, April 28-May 2, 2018.
Development of Single-Cell Expression Analysis System for Circulating Tumor Cells
Yuu Fujimura, Masayuki Ishige, Jin Akagi, Kosuke Osawa, Takahide Ino and Kazuo Takeda.
CYTO2017, B39, Boston, Massachusetts, USA, June 10–14, 2017.
Damage-Free Sorting Technique Using Disposable Microfluidic Chip for Analysis of Intracellular Interactions
Jin Akagi, Masayuki Ishige, Yuji Morishita, Yohsuke Bansho, Takahide Ino, Yuu Fujimura and Kazuo Takeda.
CYTO2017, B32, Boston, Massachusetts, USA, June 10–14, 2017.
The Emulsion Sorting Technology Platform for High-Throughput Screening Assays in Various Fields of Biology
Yohsuke Bansho, Jin Akagi, Takahide Ino, Kosuke Osawa, Tomiko Tanaka, Masayuki Ishige, Yuu Fujimura and Kazuo Takeda.
CYTO2016, B138, Seattle, Washington, USA, June 11–15, 2016.
Water-in-Oil Emulsion Sorting for Digital PCR and Single Cell Analysis Using Air Pressure Pulse Flow in Disposable Microfluidic Chip
Jin Akagi, Yuu Fujimura, Yoshie Kawase and Kazuo Takeda.
CYTO2015, B235, Glasgow, Scotland, June 26–30, 2015.
Negative Cell Sorting for Removal of Undifferentiated Cells for Regenerative Medicine
Takahide Ino, Haixin Zhang, Masayuki Ishige, Kazuhiro Aiba, Yuu Fujimura, Norio Nakatsuji and Kazuo Takeda.
CYTO2015, B242, Glasgow, Scotland, June 26–30, 2015.
A novel flow cytometry-based cell capture platform for the detection, capture and molecular characterization of circulating tumor cells
Yasuhiro Koh, Masaru Watanabe, Takeshi Sawada, Hirotsugu Kenmotsu, Masakuni Serizawa, Masayuki Ishige, Kazuo Takeda, Nobuyuki Yamamoto, Toshiaki Takahashi and Fumiaki Koizumi.
AACR2014, San Diego, CA, USA, April 5-9, 2014.
Clinical feasibility study of a novel cytometry-based-system for the detection of circulating tumor cells (CTCs) of patients with lung cance
Takeshi Sawada, Yukiko Ito, Masaru Watanabe, Yuu Fujimura, Shinji Nakamichi, Shintaro Kanda, Hidehito Horinouchi, Yutaka Fujiwara, Hiroshi Nokihara, Noboru Yamamoto, Tomohide Tamura, Yasuhiro Koh and Fumiaki Koizumi.
AACR2013, #1445, Washington, DC, USA, April 6-10, 2013.
Molecular characterization of captured circulating tumor cells (CTCs) with a novel cell sorting system
Masaru Watanabe, Takeshi Sawada, Masakuni Serizawa, Yukiko Ito, Yuu Fujimura, Fumiaki Koizumi and Yasuhiro Koh.
AACR2013, #5105, Washington, DC, USA, April 6-10, 2013.
Multivariate analysis of apoptotic markers versus cell cycle phase in living human cancer cells by microfluidic cytometry
Jin Akagi, Joanna Skommer, Anna Matuszek, Kazuo Takeda, Yuu Fujimura, Khashayar Khoshmanesh, Kourosh Kalantar-Zadeh, Arnan Mitchell, Rachel Errington,Paul J Smith, Zbigniew Darzynkiewicz and Donald Wlodkowic.
SPIE Microfluidics, BioMEMS, and Medical Microsystems XI, San Francisco, CA, USA, February 2, 2013.
Evaluation of a novel flow cytometry-based system for the detection of circulating tumor cells (CTCs)
Masaru Watanabe, Yuri Uehara, Namiko Yamashita, Yuu Fujimura, Kaori Nishio, Junko Suzuki, Fumiaki Koizumi and Yasuhiro Koh.
AACR2012, #1735, Chicago, IL, USA, March 31-April 4, 2012.
Development of disposable microfluidic chip based cell sorter for high cell purification with no damage
Yuu Fujimura and Kazuo Takeda.
CYTO2011, B144, Baltimore, MD, USA, May 21-25, 2011.
Cell clump analysis for flow cytometric enumeration of circular tumor cells by using disposable microfluidic chip
Kazuo Takeda and Namiko Yamashita.
CYTO2011, B143, Baltimore, MD, USA, May 21-25, 2011.
Expression of the Staphylococcus aureus Efflux Pumps NorA, B, and C in Response to Acidification
Que Chi Truong-Bolduc, Gilles Bolduc, Brian Celino, Jennifer Bevis, Y. Ding, Regis Villet and David Hooper.
ICAAC2010, Boston, MA, USA, September 12-15, 2010.
Development of cross-contamination free flow cytometer using a disposable multi flow channel chip with side scattered light detection optics.
Kazuo Takeda and Fumie Jimma.
CYTO2010, P227, Seattle, WA, USA, May 8-12, 2010.
Enumeration and characterization of circulating tumor cells with cross-contamination free flow cytometer
Masashi Takao, Fumie Jimma and Kazuo Takeda.
CYTO2010, P112, Seattle, WA, USA, May 8-12, 2010.
Maintenance free biosafety flow cytometer using disposable microfluidic chip (FISHMAN-R)
Kazuo Takeda and Fumie Jimma.
Clinical Cytometry 2009, Jacksonville, FL, USA, 2009
Microorganisms
Clear zone formation in microdroplets for high-throughput screening for lactic acid bacteria.
Koji Mori, Mizuki Watanabe, Keiko Nanri, Satoko Matsukura, Yuri Ota, Nobuyuki Homma, Naohiro Noda.
Frontiers in Microbiology, 15:1452573, Sep. 2024.
Single Amplified Genome Catalog Reveals the Dynamics of Mobilome and Resistome in the Human Microbiome.
Tetsuro Kawano-Sugaya, Koji Arikawa, Tatsuya Saeki, Taruho Endoh, Kazuma Kamata, Ayumi Matsuhashi, Masahito Hosokawa.
bioRxiv, Dec. 2023.
Water-in-oil droplet-mediated method for detecting and isolating infectious bacteriophage particles via fluorescent staining.
Miu Hoshino, Yuri Ota, Tetsushi Suyama, Yuji Morishita, Satoshi Tsuneda, Naohiro Noda.
Frontiers in Microbiology, 14 :1282372, Dec. 2023.
Ultrahigh-throughput screening of Trichoderma reesei strains capable of carbon catabolite repression release and cellulase hyperproduction using a microfluidic droplet platform.
Xuan Chinh Luu, Yosuke Shida, Yoshiyuki Suzuki, Daiki Kuwahara, Takeshi Fujimoto, Yuka Takahashi, Naomi Sato, Akihiro Nakamura, Wataru Ogasawara.
Bioscience, Biotechnology, and Biochemistry, 87 (11) :1393–406, Oct. 2023.
Revealing within-species diversity in uncultured human gut bacteria with single-cell long-read sequencing.
Masato Kogawa, Yohei Nishikawa, Tatsuya Saeki, Takuya Yoda, Koji Arikawa, Haruko Takeyama, Masahito Hosokawa.
Frontiers in Microbiology, 14 :1133917, Feb. 2023.
Rapid transcriptomic and physiological changes in the freshwater pennate diatom Mayamaea pseudoterrestris in response to copper exposure.
Shigekatsu Suzuki, Shuhei Ota, Takahiro Yamagishi, Akihiro Tuji, Haruyo Yamaguchi, Masanobu Kawachi.
DNA Research, 29 (6) :dsac037, Dec. 2022.
A novel high-throughput approach for transforming filamentous fungi employing a droplet-based microfluidic platform
Xuan Chinh Luu, Yosuke Shida, Yoshiyuki Suzuki, Naomi Sato, Akihiro Nakumura, and Wataru Ogasawara.
New Biotechnology, 72(25):149–58, December 2022.
Using gel microdroplets to develop a simple high-throughput screening platform for oleaginous microorganisms
Yuma Tanaka, Akihiro Nakamura, Yoshiyuki Suzuki, Kodai Maruta, Yosuke Shida, and Wataru Ogasawara.
Journal of Biotechnology, 358(10):46–54, November 2022.
Strain-level profiling of viable microbial community by selective single-cell genome sequencing.
Masahito Hosokawa, Taruho Endoh, Kazuma Kamata, Koji Arikawa, Yohei Nishikawa, Masato Kogawa, Tatsuya Saeki, Takuya Yoda, Haruko Takeyama.
Scientific Reports, 12 (1) :4443, Mar. 2022.
Propidium Iodide Staining and Flow Cytometry-Based Assessment of Heavy Metal Impact on Marine Phytoplankton
Shuhei Ota, Shigeshi Fuchida, Haruyo Yamaguchi, Takahiro Yamagishi, Hiroshi Yamamoto, Hiroshi Koshikawa and Masanobu Kawachi.
Cytologia, 87(2): 177-187, February 2022
High-throughput selection of cells based on accumulated growth and division using PicoShell particles
Mark Van Zee, Joseph de Rutte, Rose Rumyan, Cayden Williamson, Trevor Burnes, Randor Radakovits, Andrew Sonico Eugenio, Sara Badih, Sohyung Lee, Dong Hyun Lee, Maani Archang, and Dino Di Carlo.
PNAS, 119(4):e2109430119, January 2022.
7-Aminocoumarin-4-Acetic Acid as a Fluorescent Probe for Detecting Bacterial Dipeptidyl Peptidase Activities in Water-in-Oil Droplets and in Bulk
Akihiro Nakamura, Nobuyuki Honma, Yuma Tanaka, Yoshiyuki Suzuki, Yosuke Shida, Yuko Tsuda, Koushi Hidaka, and Wataru Ogasawara
Analytical Chemistry, 94(5): 2416–2424, December 2021.
Recovery of strain-resolved genomes from human microbiome through an integration framework of single-cell genomics and metagenomics
Koji Arikawa, Keigo Ide, Masato Kogawa, Tatsuya Saeki, Takuya Yoda, Taruho Endoh, Ayumi Matsuhashi, Haruko Takeyama and Masahito Hosokawa.
Microbiome, 9(1):1–16, October 2021.
Microdroplet-based system for culturing of environmental microorganisms using FNAP-sort
Kanako Saito, Yuri Ota, Dieter M. Tourlousse, Satoko Matsukura, Hirotsugu Fujitani, Masamune Morita, Satoshi Tsuneda, and Naohiro Noda.
Scientific Reports, 11:9506, May 2021.
High-throughput screening of high protein producer budding yeast using gel microdrop technology.
Hirotsugu Fujitani, Soichiro Tsuda, Tomoko Ishii, Masayuki Machida.
bioRxiv, Nov. 2019.
Differential heavy metal sensitivity in seven algal species from the NIES culture collection based on delayed fluorescence assays
Shuhei Ota, Haruyo Yamaguchi, Faustine Vanel, Shigeshi Fuchida, Hiroshi Koshikawa, Takahiro Yamagishi, Hiroshi Yamamoto, and Masanobu Kawachi.
Phycological Research, 68(1):41–49, August 2019.
Fluorescent nucleic acid probe in droplets for bacterial sorting (FNAP-sort) as a high-throughput screening method for environmental bacteria with various growth rates
Yuri Ota, Kanako Saito, Taeko Takagi, Satoko Matsukura, Masamune Morita, Satoshi Tsuneda, and Naohiro Noda.
PLOS ONE, 14(4), April 2019.
Gene
Ultrahigh-Throughput Single Emulsion Droplet Screening for the Discovery of New B Antigen Cleaving Enzymes.
Charlotte Olagnon, Jacob F. Wardman, Feng Liu, Hong-Ming Chen, Haisle Moon, Seyed A. Nasseri, Dan Seale, Peter Rahfeld, Steven J. Hallam, Jayachandran N. Kizhakkedathu, Stephen G. Withers.
ACS Catalysis, 14 (17) :12884–94, Sep. 2024.
Microfluidic generation of cell-sized liposomes using a budding strategy.
Jiajue Ji, Ryuji Kawano.
ChemRxiv, Aug. 2024.
Large-scale single-cell cloning of genome-edited cultured human cells by On-chip SPiS
Gou Takahashi and Yuichiro Miyaoka.
STAR Protoc. 4(3):102364, June 2023.
scRNA-seq analysis of hemocytes of penaeid shrimp under virus infection.
Keiichiro Koiwai, Hidehiro Kondo, Ikuo Hirono
bioRxiv, Jan. 2023.
Selection of green fluorescent proteins by in vitro compartmentalization using microbead-display libraries
Ryo Iizuka, Kentaro Tahara, Anna Matsueda, Soichiro Tsuda, Dong Hyun Yoon, Tetsushi Sekiguchi, Shuichi Shoji, and Takashi Funatsu.
Biochemical Engineering Journal, 187:108627, November 2022.
Genome editing is induced in a binary manner in single human cells
Gou Takahashi, Daiki Kondo, Minato Maeda, Yuji Morishita, and Yuichiro Miyaoka.
iScience, 25(12):105619, November 2022.
Label-free enzymatic reaction monitoring in water-in-oil microdroplets using ultra-broadband multiplex coherent anti-Stokes Raman scattering spectroscopy
Ryo Imai and Hideaki Kano.
Biomedical Optics Express, 13(3):1506, March 2022.
High-throughput screening of cell-free riboswitches by fluorescence-activated droplet sorting
Takeshi Tabuchi and Yohei Yokobayashi.
Nucleic Acids Research, 50(6):3535–50, March 2022.
Endocrine secretory granule production is caused by a lack of REST and intragranular secretory content and accelerated by PROX1
Jun Ishii, Hanako Sato-Yazawa, Korehito Kashiwagi, Kazuhiko Nakadate, Masami Iwamoto, Kakeru Kohno, Chie Miyata-Hiramatsu, Meitetsu Masawa, Masato Onozaki, Shuhei Noda, Tadasuke Miyazawa, Megumi Takagi, and Takuya Yazawa.
Journal of Molecular Histology, 53(2):437–48, January 2022.
Single-cell RNA-seq analysis reveals penaeid shrimp hemocyte subpopulations and cell differentiation process.
Keiichiro Koiwai, Takashi Koyama, Soichiro Tsuda, Atsushi Toyoda, Kiyoshi Kikuchi, Hiroaki Suzuki, Ryuji Kawano.
eLife, 10 :e66954, Jun. 2021.
The risk ofunintentional propagation of poliovirus can be minimized by using human cell lineslackingthefunctional CD155 gene
Satoshi Koike.
Microbiology and Immunology, 64(12): 835–839, September 2020.
Monitoring circulating tumor DNA revealed dynamic changes in KRAS status in patients with metastatic colorectal cancer
Yuji Takayama,1 Koichi Suzuki, Yuta Muto,1 Kosuke Ichida, Taro Fukui, Nao Kakizawa, Hideki Ishikawa, Fumiaki Watanabe, Fumi Hasegawa, Masaaki Saito, Shingo Tsujinaka, Kazushige Futsuhara, Yasuyuki Miyakura, Hiroshi Noda, Fumio Konishi, and Toshiki Rikiyama.
Oncotarget, 9(36): 24398–24413, May 2018.