Reagents - Microfluidic Chip-Based Gentle Cell Sorter, Single Cell & Cluster Dispenser | On-chip Bio

Reagents

List of reagents used for On-chip products

Cell sorter applications

Sheath fluid

Ready-to-use sheath fluids are available for purchase. These contain the anti-cell adhesion reagent, Through Path Plus. Sheath fluids are used to run the cell sorter.

Through Path Plus 40×(10mL x 6 bottles/pack)

吸着防止

Catalog number 2001004-6
Product name Through Path Plus 40×
Purpose of use Coats microchannel and reservoirs of the microfluidic chip to prevent cell adhesion and thus cell loss
Usage Dilute 40-folds with culture medium or buffer (e.g., PBS)
Storage condition 4℃ (use within 1 month after opening)
Quantity 10 mL x 6 bottles/pack
Remarks We have confirmed that it is non-toxic to most normal cells

On-chip T buffer (500 mL)

Catalog number 2001014
Product name On-chip T buffer
Purpose of use Ready-to-use PBS-based buffer to suspend cells and used as sheath fluid. It contains Through Path Plus to allow coating of microchannel and reservoirs of the microfluidic chip, pipette tips and tubes to prevent cell adhesion, and thus cell loss
Usage Use as is
※Please open on a clean bench since the composition is similar to culture medium
Storage condition 4℃ (use within 1 month after opening)
Quantity/td> 500mL
Remarks We have confirmed that it is non-toxic to most normal cells

Sample buffer

We supply buffers used to suspend cells. Those buffers contain additives to reduce the rate of cell sedimentation. They are ready-to-use, and they come in 2x solution and powder form.

On-chip Sample Buffer 2×(50 mL)

On-chip sample buffer 2X

Catalog number 2001015
Product name On-chip sample buffer 2×
Purpose of use Ready-to-use serum-free cell culture medium used to suspend cells for sorting. It contains Through Path Plus to allow coating of microchannel and reservoirs of the microfluidic chip to prevent cell adhesion and thus cell loss, and viscosity agent for prolonged cell suspension
Usage Dilute 1:1 with sample
Storage condition 4℃ (use within 1 month after opening)
Quantity 50mL
Remarks Dilute 1:1 with sample as the viscosity adjuster is included in the 2x concentration. Changes in viscosity will reduce sorting efficiency

On-chip Sample Buffer Production Kit

Catalog number 2001011
Product name On-chip Sample Buffer Production Kit
Purpose of use A kit to produce Sample buffer with your preferred media. This is best for when using sensitive cells or special medium. It does not contain Through Path Plus, and it can be added as required
Usage Dissolve this product in 15mL of medium to be used as Sample buffer, or dissolve in 7.5mL of medium to be used as Sample buffer 2x equivalent
Storage condition 15-25°C (After dissolving, keep refrigerated and use within 2 weeks)
Quantity 3 packs to produce 15 mL Sample buffer each
Droplet applications

Catalog number 2003003
Product name On-chip®️ FluoroSurfactant-5wtH Oil
Quantity 10mL/bottle

Catalog number 2003004
Product name On-chip®️ FluoroSurfactant-0.1wtH Oil
Quantity 100mL/bottle
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