Reagents
List of reagents used for On-chip products
Cell sorter applications
Sheath fluid
Ready-to-use sheath fluids are available for purchase. These contain the anti-cell adhesion reagent, Through Path Plus. Sheath fluids are used to run the cell sorter.
Through Path Plus 40×（10mL x 6 bottles/pack)
| Catalog number | 2001004-6 |
| Product name | Through Path Plus 40× |
| Purpose of use | Coats microchannel and reservoirs of the microfluidic chip to prevent cell adhesion and thus cell loss |
| Usage | Dilute 40-folds with culture medium or buffer (e.g., PBS) |
| Storage condition | 4℃ (use within 1 month after opening) |
| Quantity | 10 mL x 6 bottles/pack |
| Remarks | We have confirmed that it is non-toxic to most normal cells |
On-chip T buffer (500 mL)
| Catalog number | 2001014 |
| Product name | On-chip T buffer |
| Purpose of use | Ready-to-use PBS-based buffer to suspend cells and used as sheath fluid. It contains Through Path Plus to allow coating of microchannel and reservoirs of the microfluidic chip, pipette tips and tubes to prevent cell adhesion, and thus cell loss |
| Usage | Use as is ※Please open on a clean bench since the composition is similar to culture medium |
| Storage condition | 4℃ (use within 1 month after opening) |
| Quantity/td> | 500mL |
| Remarks | We have confirmed that it is non-toxic to most normal cells |
Sample buffer
We supply buffers used to suspend cells. Those buffers contain additives to reduce the rate of cell sedimentation. They are ready-to-use, and they come in 2x solution and powder form.
On-chip Sample Buffer 2×（50 mL)
| Catalog number | 2001015 |
| Product name | On-chip sample buffer 2× |
| Purpose of use | Ready-to-use serum-free cell culture medium used to suspend cells for sorting. It contains Through Path Plus to allow coating of microchannel and reservoirs of the microfluidic chip to prevent cell adhesion and thus cell loss, and viscosity agent for prolonged cell suspension |
| Usage | Dilute 1:1 with sample |
| Storage condition | 4℃ (use within 1 month after opening) |
| Quantity | 50mL |
| Remarks | Dilute 1:1 with sample as the viscosity adjuster is included in the 2x concentration. Changes in viscosity will reduce sorting efficiency |
On-chip Sample Buffer Production Kit
| Catalog number | 2001011 |
| Product name | On-chip Sample Buffer Production Kit |
| Purpose of use | A kit to produce Sample buffer with your preferred media. This is best for when using sensitive cells or special medium. It does not contain Through Path Plus, and it can be added as required |
| Usage | Dissolve this product in 15mL of medium to be used as Sample buffer, or dissolve in 7.5mL of medium to be used as Sample buffer 2x equivalent |
| Storage condition | 15-25°C (After dissolving, keep refrigerated and use within 2 weeks) |
| Quantity | 3 packs to produce 15 mL Sample buffer each |
Droplet applications
| Catalog number | 2003003 |
| Product name | On-chip®️ FluoroSurfactant-5wtH Oil |
| Quantity | 10mL/bottle |
| Catalog number | 2003004 |
| Product name | On-chip®️ FluoroSurfactant-0.1wtH Oil |
| Quantity | 100mL/bottle |
