We are pleased to announce an upcoming webinar featuring research from the University of British Columbia (UBC), Canada, exploring a powerful new approach to identifying microbial enzymes involved in N-glycan degradation.
Webinar Title
Uncovering Microbial N-Glycan Degradation Pathways Through Ultrahigh-Throughput Single Emulsion Droplet Screening
Presenters: Rajneesh K. Bains, Charlotte Olagnon, Jacob F. Wardman, and Stephen G. Withers
Affiliation: Department of Chemistry, University of British Columbia, Canada
Advancing Enzyme Discovery with Droplet Microfluidics
N-glycans play essential roles in biological systems, yet discovering enzymes capable of degrading their complex structures remains challenging. Traditional functional metagenomic screening approaches often rely on simplified substrates that do not accurately reflect the structural complexity of naturally occurring glycans.
To overcome this limitation, the UBC research team developed an innovative fluorescence-quenched N-glycan probe that produces a fluorescent signal when cleaved by enzymes acting on linkages within complex N-glycans.
By combining this novel probe with an ultrahigh-throughput droplet-based screening platform, the researchers have established an efficient approach to identifying N-glycan-degrading enzymes and pathways from diverse metagenomic libraries.
Accelerating Screening with On-chip Droplet Selector
A key component of this screening workflow is On-chip Droplet Selector, which enables fluorescence-based selection of droplets during the screening process.
By identifying and selecting droplets exhibiting fluorescence signals associated with enzymatic activity, Droplet Selector supports the efficient screening of large metagenomic libraries for promising enzyme candidates.
The droplet-based approach also dramatically reduces substrate consumption, an especially important advantage when working with complex, synthetically demanding glycan probes.
This research demonstrates how combining innovative substrate design with advanced droplet sorting technology can help overcome conventional screening limitations and expand opportunities for discovering novel enzymes.
Join the Webinar!
In this webinar, the UBC research team will share their approach to developing fluorescence-responsive N-glycan probes, integrating them into an ultrahigh-throughput droplet screening workflow, and exploring microbial N-glycan degradation pathways.
Participants will gain insights into how On-chip Droplet Selector can be incorporated into fluorescence-based enzyme screening workflows, offering new possibilities for functional metagenomics, glycoside hydrolase discovery, and microbial enzyme research.
Whether you are working in enzyme discovery, glycobiology, functional metagenomics, or droplet microfluidics, this webinar offers an opportunity to explore practical applications of ultrahigh-throughput droplet screening.
Don’t miss this opportunity to learn how droplet microfluidics is advancing the discovery of novel microbial enzymes!
Register now to secure your spot!
Date and Time: October 21, 2pm PST | 5pm EST
Don’t miss the opportunity to gain valuable knowledge and connect with fellow researchers at the forefront of glycan biology!