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https://on-chipbio.com/document/
Title: Efficient Discovery of B Antigen-Cleaving Enzymes Using Ultrahigh-Throughput Droplet Microfluidics
Charlotte Olagnon, Jacob F. Wardman, and Stephen G. Withers, et al. Department of Chemistry, University of British Columbia
Introduction:
ABO blood type mismatch is a critical challenge in blood transfusion. B-type red blood cells (RBCs) carry B antigens, and transfusions from a B-type donor to an incompatible recipient can lead to immune reactions, potentially resulting in death. The goal is to convert B-type RBCs to universal O-type RBCs by removing the terminal sugar (galactose) from the B antigen. Previous enzyme-based methods for this conversion have either been inefficient or required impractical amounts of enzyme. This study presents a droplet-based microfluidic screening system to discover new enzymes from metagenomic libraries capable of cleaving the B antigen efficiently.