SINGLE CELL DISPENSER : On-chip SPiS - Microfluidic Chip-Based Gentle Cell Sorter, Single Cell & Cluster Dispenser | On-chip Bio

SINGLE CELL DISPENSER : On-chip SPiS

Gentle, automated and accurate single cell dispenser

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On-chip’s Single Particle isolation System (SPiS) enables accurate dispensing of single to multiple particles into wells of 96- and 384-well plates.

Dispensing technology

The operation of On-chip SPiS is straightforward. It has an inbuilt auto-dilution function to adjust the sample to its optimal concentration. A very small aliquot (<0.3 µL) of the sample is aspirated by a dispensing pipette tip. CCD camera counts the number of cells present inside this aliquot by image recognition. When a specified number of cell(s) is detected, the aliquot is dispensed out into a well of a 96- or 384-well plate. Otherwise, it is dispensed back into the sample tube (or discarded if necessary), and the instrument repeats this process until the number of cell(s) match the specified cell count. On-chip SPiS ensures that almost every well contains the desired cell number. On-chip SPiS can plate single cell per well accurately and easily.

Features

Dispensing of small to large particles

On-chip SPiS allows dispensing of particles sized between 7 µm and 200 µm.

Compact and easy operation

On-chip SPiS is small enough to fit inside a biosafety cabinet and it is easy to operate.

Inbuilt auto-dilution function and CCD camera image recognition

Concentration determination and automatic dilution program adjusts the sample to optimal concentration without having to do a complex dilution calculation. Cells within the pipette tip are counted using a CCD camera for accurate single cell deposition into wells.

Freedom of Buffers

Any buffers of your choice can be used, such as water, culture medium and oil.

Specifications

Product Name: On-chip SPiS
Catalog number 70001
Device configuration Main unit, control PC (Windows)
Instrument size 610mm x 365mm x 450mm (W x D x H)
Dispensing method Disposable pipette tips
Recognition method Image recognition by 5 megapixel CMOS sensor camera
Dispensing volume 0.1~0.3μL (adjustable)
Sample Cells, pollen, protists, cell clusters, microbeads, etc.
Recognizable sizes 7-200μm (confirmed with cells), >3μm (microbeads etc.)
Dispensing accuracy >90% (depends on sample)
Biosafety Fit inside a biosafety cabinet
Damage Low damage to cells etc.
Sample agitation Automated resuspension by disposable pipette tips
Buffer Water, culture media, sea water, etc.
Handling speed 60 min/96 wells and 200 min/384 wells (Reference time only. Depends on sample conditions)
Supported well plate 96 and 384 well plate

Consumables

On-chip SPiS dispensing tips

Sterile dispensing pipette tips designated for On-chip SPiS.

Product name Chip-384S
Catalog number 1007001
Quantity 384 tips per pack
Material Polypropylene
Storage condition Room temperature
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